Collection of Cell Lines in Veterinary Medicine (CCLV)
Department of Experimental husbandry and Biorisk Management
Short Description
The Collection of Cell Lines generates, characterises and collects cell lines which are of interest for veterinary research and diagnostics. Currently the collection comprises 1,270 lines. It includes cell lines of farm animals such as cattle, pig, horse, sheep, goat and poultry and other mammals, birds, reptiles, fishes and insects (70 species).
Authentication and quality-control tests (tests for contamination with mycoplasms, bacteria, BVDV) of the cultures are done routinely.
Cell cultures are provided to scientists of the Friedrich-Loeffler-Institute and other non-commercial institutions worldwide after consultation of a scientific staff member of the CCLV.
Identity Controls
Species determination is done by:
- Sequencing
- PCR-RFLP
- Proteomics
- PCR (species specific)
- taxon specific indirect immunofluorescence
Genotyping of bovine, porcine and human cell lines is done by STR analysis.
Controls of the lines showed misidentification in some cases. Details are indicated in the cell line passport.